In recent decades, Border Studies have gained importance and have seen a noticeable increase in development. This manifests itself in an increased institutionalisation, a differentiation of the areas of research interest and a conceptual reorientation that is interested in examining processes. So far, however, little attention has been paid to questions about (inter-)disciplinary self-perception and the methodological foundations of Border Studies and the associated consequences for research activities. This thematic issue addresses these desiderata and brings together articles that deal with their (inter-)disciplinary foundations as well as method(olog)ical and practical research questions. The authors also provide sound insights into a disparate field of work, disclose practical research strategies, and present methodologically sophisticated systematizations.